Advanced Bioimaging Support (ABiS)

Detailed structures of cultured cells and so on with the sample thickness of several microns can be determined by cryo-microscopy using a high-voltage electron microscope. The native structures of biological specimen can be observed by rapid freezing to trap them in amorphous ice at millisecond order. For reconstruction of the structures by electron tomography and single particle analysis, we support the data collection by cryo-electron microscopy. By electron tomography, the cell structures at nano-meter resolution can be determined. By single particle analysis, the atomic structures of biological complexes with high molecular weight can be determined.